COMPETITIVE BINDING OF ATP AND ACRIDINE ORANGE BY MUSCLE
نویسندگان
چکیده
منابع مشابه
Competitive Binding of Atp and Acridine Orange by Muscle.
Introduction.-The lifetime of electronic singlet excitation in molecules is too short to allow the utilization of these excitational energies in biological systems.' Triplet excitations last longer, but the probabilities of their occurrence are small. Circumstances which increase the probability of these transitions may thus have biological importance. It has been shown' that dyes and certain o...
متن کاملAcridine Orange Binding by Micrococcus Lysodeikticus.
Beers, Roland F., Jr. (Johns Hopkins University, Baltimore, Md). Acridine orange binding by Micrococcus lysodeikticus. J. Bacteriol. 88:1249-1256. 1964.-Micrococcus lysodeikticus cells bind acridine orange (AO) reversibly. The adsorption isotherm is consistent with a highly cooperative-type binding similar to that observed with polyadenylic acid. The cells exhibit a strong buffering action on t...
متن کاملCarotenoid pigments and photokilling by acridine orange.
It has long been known that certain organic dyes can photosensitize cells, leading to cellular damage or death. Recently, studies were performed on one such dye, Toluidine Blue, to determine the mechanism of its lethal photosensitization of bacteria (M. M. Mathews, J. Bacteriol. 85:322, 1963). These studies showed that the cellular site of the lethal action was the cytoplasmic membrane and its ...
متن کاملInvestigating the binding of acridine, acridine orange, and acridine yellow G to humic acid through fluorescence quenching.
A fluorescence quenching method was used to determine the equilibrium binding constants for the association of acridine, acridine orange, and acridine yellow G to humic acid. The fluorescence of each polycyclic aromatic nitrogen heterocycle (PANH) was monitored as aliquots of humic acid were added, and a Stern-Volmer plot was produced in which the slope is the equilibrium constant of the bindin...
متن کاملInhibition and activation of polynucleotide phosphorylase by acridine orange.
Preliminary studies by Beers et a2. (1) have shown that the zinc salt of acridine orange can either inhibit or accelerate the rate of polymerization of polynucleotides by polynucleotide phosphorylase of Micrococcus lysodeileticus, depending upon the experimental conditions. We have now confirmed these findings with the zinc-free dye. Some evidence for a reversal of inhibition with degraded ribo...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1957
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.43.5.373